Some easy summarized reading - While most veggies are known for vitamins and other macronutrients rather than protein, a few high protein vegetables can significantly boost your daily intake. [🍲High Fiber ‘HF’; 🥔High Protein ‘HP] Daily Goal: 2-3 cups, ugh.. 😉 These nutrient-dense cooked options, offer a little more than just protein per cup. Here are 10 high-protein vegetables to add to your meal planning: 1. 🫘Chickpeas: one cup of cooked chickpeas, you can find 14.53 grams of protein, sauteed, cooked, salad. 2. 🫘 Edamame (Soybeans): HF, HP - A complete protein with approximately 11–18.5 grams per cup, (a complete protein) - snacks / stir-fries. 3. 🫘 Lentils: Technically a legume, but a staple with 11 grams of protein per cup, (potassium, iron, fiber) - salads / soups. 4. 🥗 Green Peas: Provides about 8 grams of protein per cup, heart n gut healthy fiber, pasta / rice. 5. 🥔Russet Potato: large potato w skin (baked), 7.86 grams per cup. 6. 🥬Spinach: HF, HP - When cooked, provides roughly 5.3 grams of protein per cup. Takes about 5 cups of raw spinach to equal 1 cooked cup. 7. 🧄Brussels Sprouts: HF, HP - Provides nearly 3-4 grams of protein per cup (cooked), Vit C, side dish. 8. 🌞Asparagus: HF, HP - Contains approximately 3–4.3 grams of protein per cup, rich in vitamins C and K. 9. 🥦Broccoli: Provides about 4 grams of protein per cup (cooked), great source of fiber and vitamins. 10. 🌽Sweet Corn: HF, HP - A large ear of corn provides around 4 grams of protein, (Vit Bs, antioxidants). ‼️Other notable mentions: Mushrooms (specifically Portobello), Lima beans, and Collard greens. 👉Daily Fillers: Cucumber, Zucchini – Have less than 2g per cup, focusing more on hydration and micronutrients. 🗒️Note: For Maximum PROTEIN protected intake, enjoy them STEAMED, ROASTED, STIR-FRIED. DO NOT BOIL – no nutrients contained.

Hi all. Am curious if folks have protocols to improve mitochondrial efficiency without using peptides. Ie eating sardines, taking coq10, ensuring 7-9 hours of sleep etc. Would be very interested in understanding how Folks think about what they are taking (intended effect), timing and dosage. I saw a few historical posts touching on this but nothing that got into the details Of the why and how.

What protocol do you follow to minimize jet lag, avoid drops in HRV, and improve sleep after traveling to a different time zone? @Anthony Castore

Peptides are often treated like supplements you can stack for convenience. One for repair, one for metabolism, one for inflammation. That mindset leads people to assume they can simply mix peptides in the same syringe and inject once. The problem is peptides are not pills. They are fragile, information-carrying molecules whose behavior is governed by physics, chemistry, and biology at the same time. A peptide is not just a chain of amino acids. In solution it exists as a three-dimensional structure held together by weak forces like hydrogen bonds, electrostatic interactions, and hydrophobic effects. These forces are highly sensitive to the environment. Small changes in pH, ionic strength, or solvent conditions can change the peptide’s shape, stability, and behavior. When a peptide is manufactured, it is stabilized in a very specific formulation. That formulation controls pH, charge, ion balance, and solubility so the peptide stays folded correctly and remains biologically active. When you mix two peptides together, you destroy that controlled environment and create a new, untested chemical system. One of the first things that goes wrong is charge balance. Peptides carry electrical charge depending on pH. That charge helps keep molecules from sticking to each other. Mixing peptides can shift pH just enough to reduce repulsion between molecules. When repulsion drops, attraction wins, and peptides begin to stick together. Ionic strength matters too. Mixing solutions often increases ion concentration, which compresses the electrical “buffer” that keeps peptides apart. This allows molecules to drift close enough for hydrophobic regions to interact. Water dislikes exposed hydrophobic surfaces, so peptides clump together to lower free energy. This is basic solution physics. Once aggregation starts, it accelerates. A few misfolded molecules form a nucleus, which seeds further aggregation. Early clumps may be invisible, but they still matter. They reduce the amount of active peptide, alter absorption, and change signaling behavior.

In the latest DDT Method podcast with @Anthony Castore , Anthony discussed NAD supplementation. What I found interesting is that Anthony seemed to be against NAD supplementation via NAD+ and its precursors if I understood correctly, and a more appropriate approach would be to instead use a combination of 5 amino and 1MNA. He suggested using 5 amino pre workout and 1MNA on rest days in the evening. Anthony - would you be able to expand on your thoughts regarding NAD supplementation given it seems like a given in the longevity community that you supplement with NAD+ (injection or IV) or its precursors (NMN, NR).