For the most part standard distilled water works great but when you're dealing with expensive seeds and you can't afford failure, sterile water has proven time and time again to be 100% more successful

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This ready-to-use content is designed for your educational course on cannabis tissue culture (micropropagation). It includes a suggested course outline with 8 modules, key learning objectives, detailed explanations, practical tips, and visuals for engagement. Feel free to adapt it for videos, slides, quizzes, or hands-on labs. The material is based on established protocols (e.g., MS or DKW media, meristem culture) and industry best practices as of 2026. Course Overview Title: Mastering Cannabis Tissue Culture: From Lab Basics to Commercial Scalability Target Audience: Beginners to intermediate growers, cultivators, breeders, and industry professionals. Duration: 8-12 weeks (self-paced or instructor-led). Objectives: Understand the science, benefits, and hands-on techniques of tissue culture to produce disease-free, genetically consistent cannabis plants. Prerequisites: Basic plant biology knowledge; access to a simple lab setup recommended for practical modules. Module 1: Introduction to Tissue Culture in Cannabis • Key Topics: • What is tissue culture? (In vitro propagation using small explants in sterile media). • History: From traditional cloning/seeds to modern micropropagation. • Why cannabis? Addresses issues like Hop Latent Viroid (HLVd), genetic drift, and scalability. • Benefits: • Disease-free plants (eliminates viroids, viruses, pests). • Genetic consistency (true-to-type clones with identical THC/CBD/terpene profiles). • Space efficiency (thousands of plants from one explant in minimal space). • Year-round production and strain preservation. Module 2: Lab Setup and Sterile Techniques • Essential Equipment: • Laminar flow hood (for sterile work). • Autoclave/pressure cooker (sterilization). • Vessels (jars, tubes), scalpel, forceps, pH meter, growth shelves. • Home setup cost: Under $500; commercial: $10k+. • Sterility Best Practices: • Work in a clean room; use 70% ethanol, bleach. • PPE: Gloves, mask, lab coat. • Common contamination sources: Bacteria, fungi, endophytes.

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