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Tissue Culture Mastery Hub

6 members • $9/month

5 contributions to Tissue Culture Mastery Hub
Bleach vs pesticides for de contamination
Bleach (sodium hypochlorite) is generally more effective and widely recommended than pesticides (including fungicides or bactericides) for general lab surface sterilization and grow room decontamination. Pesticides are designed primarily for controlling pests or pathogens on living plants, not for broad disinfection of inanimate surfaces. Why Bleach Outperforms Pesticides for Disinfection - Broad-spectrum action — Bleach rapidly kills bacteria, fungi, viruses, and many spores by oxidizing cellular components. Standard 10% dilutions (from household bleach) are highly effective on benches, tools, floors, and equipment in tissue culture labs and grow rooms. - Pesticides’ limitations — Fungicides (e.g., bavistin, mancozeb) or bactericides target specific organisms on plants and lack the broad, rapid kill needed for surfaces. They may leave residues that inhibit cultures or are ineffective against diverse contaminants. - Standard protocols — In tissue culture labs, bleach is the go-to for wiping surfaces and decontaminating waste. In grow rooms/greenhouses, bleach or similar oxidizers are used for between-cycle cleanouts to eliminate mold, bacteria, and pathogens. Here are examples of bleach being used to wipe lab benches in tissue culture settings: 3 “LARGE” 4 “LARGE” 5 “LARGE” 6 “LARGE” 7 “LARGE” 8 “LARGE” Spraying disinfectants (often bleach-based or similar) during grow room/greenhouse decontamination: 9 “LARGE” 10 “LARGE” 11 “LARGE” 12 “LARGE” In contrast, pesticides/fungicides are applied directly to plants for targeted control: 0 “LARGE” 1 “LARGE” 2 “LARGE” Occasionally, low concentrations of fungicides are added as pre-treatments for tough explants or to supplement sterilization, but they’re not a replacement for bleach on surfaces. Bleach Alternatives (If Needed) For less corrosive options: 70% ethanol, quaternary ammonium compounds (e.g., Green-Shield), or hydrogen peroxide-based products (e.g., ZeroTol). These are effective but bleach remains the most reliable and cost-effective for thorough decontamination.
0 likes • 3d
This is great information! I am going to get this done this weekend. My plants are defintiely much lighter now and the soil is starting to dry out, but they look very healthy. I need to ask you about watering.
Welcome!! Introduce yourself
Welcome to Tissue Culture Mastery! This is the go-to community for building profitable, efficient, and reliable tissue culture skills – from beginner home setups to commercial-scale labs. We provide proven protocols, business strategies, live training, and real solutions so you can eliminate failures, scale production, and unlock serious revenue potential. Our vision: A world where everyone has the tools and knowledge to reach their full potential in tissue culture. Join the conversation, share your progress, and let’s grow elite plants – together. 🌱 what is your level of interest in tissue culture ?
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3 members have voted
0 likes • 4d
I'm a total beginner with tissue culture. I'm even terrified to cut clones off these beautiful plants, but I have been watching a ton of videos and I think I will be okay. I have quite a bit of grow space. You talk a lot about contamination....Should I bug bomb my grow room? My dogs do not go in there and the only time I have ever gotten mites was from bringing clones in.
Class is coming are you in ? Dec 18-19
In this exclusive 2-day LIVE webinar, I’m pulling back the curtain – with a real student right beside me – as we go from sterile setup to rooted plantlets… step by step, in real time. You’ll watch live demos of: • Rockwool cloning mastery (95%+ rooting rates) • Tissue culture from explant to multiplication (virus-free elites!) • Sterilization protocols that kill contamination dead • Media recipes, hormones, and environmental tweaks for explosive growth Plus: Unlimited live Q&A – ask anything, get answers on the spot. Troubleshoot your setup. Fix your failures. No recorded fluff – this is hands-on, interactive training over two full days. Spots are limited for live interaction – don’t miss your chance to transform from frustrated cloner to propagation pro. Register now and lock in your seat:
1 like • 4d
Can I get in on this?
1 like • 4d
@Blake Barber Me!!!???
Meristem
When in doubt .. cut it out 🔬🔪 If you suspect problems .. meristem are the solution Removing the body tends to eliminate most problems, however it's not always easy sometimes it can require hundreds of pieces to find one that's not infected
Meristem
0 likes • 4d
Last time I sprouted seeds..I usually get feminized, and now and then I toss out a male, but last time there was a hermie that I didn't catch and all my nice bud had major seeds in it. Bummer.
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Elizabeth Olson
1
3points to level up
@elizabeth-olson-7669
CannaEnthusiast

Active 3d ago
Joined Dec 17, 2025
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