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Tissue Culture Mastery Hub

6 members • $9/month

6 contributions to Tissue Culture Mastery Hub
Why sterile water ?
For the most part standard distilled water works great but when you're dealing with expensive seeds and you can't afford failure, sterile water has proven time and time again to be 100% more successful
Why sterile water ?
2 likes • 17d
I never considered the difference this would make. Thank you for sharing šŸ’š
Multiple ChoicešŸ¤“
2. Which of the following is a natural auxin commonly used in tissue culture media? a) Kinetin b) Indole-3-acetic acid (IAA) c) Gibberellic acid (GA3) d) Zeatin
1 like • Jan 7
B
Multiple Choice
What is the most essential piece of equipment for maintaining sterility in tissue culture? a) Autoclave b) Laminar flow hood c) pH meter d) Growth shelves
1 like • Jan 6
B
Merry Christmas šŸŽ„ā¤ļø
Tissue culture is the future 2026šŸšŸ˜‰
Merry Christmas šŸŽ„ā¤ļø
0 likes • Dec '25
Merry Christmas šŸ’š
Ideal water conditions
Hey kid, imagine your cannabis plant is like a sponge that lives in a pot of dirt (we call it soil). It needs water to drink and grow big and strong, but it also needs air to breathe through its roots. If the soil is always soggy like a wet towel, the roots can’t breathe and might get sick (that’s overwatering). If it’s super dry like sand in a desert, the plant gets thirsty and sad (that’s underwatering). The perfect moisture is like a wrung-out sponge—not dripping wet, but not bone-dry either. The top couple inches of soil should feel dry, but deeper down it’s still a bit damp. This is the ā€œwet-dry cycleā€: You water it good so the whole pot gets soaked (wet phase—roots drink up nutrients), then you wait and let the top dry out (dry phase—roots get fresh air and grow stronger looking for water). Why this cycle is awesome - Wet time: Plant slurps water and food. - Dry time: Roots breathe oxygen and stretch out bigger. Most growers water when the top 1-2 inches of soil is dry (stick your finger in like testing cake batter—if it comes out clean and dry, time to water). Don’t water every day or on a schedule—check the plant! Signs your plant is telling you something Overwatered (too wet, no air): Leaves droop heavy, turn yellow, soil smells bad. 0 ā€œLARGEā€ 1 ā€œLARGEā€ 2 ā€œLARGEā€ 3 ā€œLARGEā€ Underwatered (too dry): Leaves wilt and curl up, tips get crispy brown. Healthy happy plant: Perky green leaves, standing tall. 10 ā€œLARGEā€ 11 ā€œLARGEā€ Best ways to check (super easy, no fancy tools needed) 1. Finger test — Poke your finger into the soil about as deep as your first knuckle (or two). Feels dry? Water! Feels wet or muddy? Wait a day or two. 7 ā€œLARGEā€ 8 ā€œLARGEā€ 9 ā€œLARGEā€ 2. Lift the pot — Right after watering, lift it—feels heavy like a full backpack. When it’s ready for more water, it’ll feel light like an empty one. 4 ā€œLARGEā€ 5 ā€œLARGEā€ 6 ā€œLARGEā€ Practice these every day. Soon you’ll know just by looking or lifting when it’s thirsty. Water slowly until some drips out the bottom, then stop. Your plant will thank you with big healthy growth! Start small, watch it closely, and you’ll get the hang of it quick.
1 like • Dec '25
I’ve also ran into pH issues due to overwatering
1-6 of 6
David Rivera
2
15points to level up
@david-rivera-1642
Apprentice in the making

Active 4d ago
Joined Dec 13, 2025
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