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8d (edited) • Peptide Tips
How to Reconstitute Buffered NAD+ (250mg) or (500mg)
This post is for research and educational purposes only — not medical advice and not intended for human consumption.
I've been getting a lot of questions on NAD+ reconstitution, so let me break this down as simply as possible.
What You're Working With
- 1 vial of Buffered NAD+ — 250mg
- 2.5mL of bacteriostatic water
The Math
2.5mL of bacteriostatic water = 250 units on an insulin syringe.
You have 250mg of NAD+ dissolved in 250 units. That makes the math incredibly clean:
1 unit = 1mg of NAD+
That's it. Every single unit on your syringe equals exactly 1mg.
So if you want to measure out common research doses, here's how it looks:
- 10mg = 10 units (0.1mL)
- 25mg = 25 units (0.25mL)
- 50mg = 50 units (0.5mL)
- 75mg = 75 units (0.75mL)
- 100mg = 100 units (1.0mL)
No calculators needed. Whatever dose you're researching, the number of units is the same number as the milligrams.
Reconstitution Tips
NAD+ is light-sensitive, so if your vial isn't already amber, store it somewhere dark or wrap it in aluminum foil after reconstitution. Keep it refrigerated at all times and use it within about 14 days once reconstituted.
Why 2.5mL?
This is a small 3mL vial, so 2.5mL is about as much bacteriostatic water as you can comfortably fit while still leaving room to draw from it. I use 2.5mL specifically because it makes the math 1:1. You could use more or less bacteriostatic water, but then you'd need to calculate your units per milligram every time. With 2.5mL, you never have to think about it — units and milligrams are the same number. Keep it simple.
What If You Have a 500mg Vial?
Same idea, same size vial. With a 500mg vial in a 3mL container, you'd use the full 3mL of bacteriostatic water.
3mL = 300 units. You have 500mg in 300 units.
500mg ÷ 300 units = 1.67mg per unit.
So every 10 units (0.1mL) = 16.7mg of NAD+.
Here's how common research doses look with 3mL:
- 25mg = 15 units
- 50mg = 30 units
- 75mg = 45 units
- 100mg = 60 units
Not as clean as the 1:1 math on the 250mg vial, but still straightforward once you know every 10 units gives you roughly 16.7mg. And if you ever want to double-check your math or play around with different reconstitution volumes, you can always use the calculator at peptideprice.store/calculator.
That's all there is to it. Clean math, easy protocol. If you have questions, drop them below 💪
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How to Reconstitute Buffered NAD+ (250mg) or (500mg)
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