24h • Muscle Building
🧬💉🧪Reconstituting IGF-1 LR3💪💉🧬
In this in-depth video tutorial, I walk you through the complete process of reconstituting a 1mg vial of IGF-1 LR3 (Insulin-like Growth Factor 1 Long R3), a synthetic peptide analog of human IGF-1 with an extended half-life due to its arginine substitution at position 3 and a 13-amino acid extension at the N-terminus. This peptide is widely discussed in scientific research, bodybuilding, and biohacking circles for its potential roles in promoting muscle hypertrophy, enhancing recovery from exercise, supporting fat loss, and aiding in cellular repair and growth factor signaling pathways. However, it’s important to note that IGF-1 LR3 is strictly for laboratory and research use, not approved for human consumption by regulatory bodies like the FDA.
We use a combination of 0.6% acetic acid (AA) for initial dissolution—since IGF-1 LR3 is sensitive to neutral pH and can degrade in plain water—followed by bacteriostatic water (BAC) for dilution and preservation, which contains benzyl alcohol to inhibit bacterial growth. This method ensures stability and sterility, resulting in a final concentration of 500mcg/mL in a total volume of 2mL, making it easier for precise dosing in research settings.
Key steps covered in detail:
- Preparation and Supplies Gathering: Start by assembling all necessary items in a clean, sterile workspace. You’ll need a 1mg vial of lyophilized (freeze-dried) IGF-1 LR3 powder, 0.6% acetic acid solution, bacteriostatic water, large 3 mL syringess, alcohol wipes or isopropyl alcohol for sanitizing and gloves to maintain hygiene. Double-check expiration dates and ensure everything is stored at appropriate temperatures—peptides like this should be kept frozen or refrigerated until use.
- Calculating the Dilution Ratio: For a 1mg vial aiming for 500mcg/mL, we’ll use 0.5mL of AA initially to dissolve the powder, followed by 1.5mL of BAC, totaling 2mL. This concentration allows for easy micro-dosing; for example, 0.1mL would deliver 50mcg. Always verify your math based on your specific research needs—adjust volumes if targeting a different strength, but avoid over-diluting to prevent instability.
- Initial Dissolution with Acetic Acid: Sanitize the vial tops with alcohol wipes. Using a sterile syringe, draw up exactly 0.5mL of 0.6% AA. Insert the needle into the vial at angle along the side of the IGF-1 LR3 vial to let the liquid trickle down gently—do not inject directly onto the powder to minimize foaming or agitation, which could denature the peptide and reduce its potency. Allow the powder to dissolve naturally at room temperature for 1-2 minutes; gently roll or swirl the vial if needed, but never aggressively shake as this can break peptide bonds.
- Adding Bacteriostatic Water for Final Dilution: Once fully dissolved (the solution should be clear with no visible particles), draw up 1.5mL of BAC water with a new sterile syringe. Inject it slowly into the vial in the same gentle manner. The AA helps with solubility, while BAC extends shelf life by preventing microbial contamination. Swirl lightly to mix—again, no shaking—and let it sit for a few minutes to ensure homogeneity.
- Final Mixing, Inspection, and Storage: Inspect the solution for clarity; if cloudy, it may indicate improper handling. Label the vial (or write down) with the date, concentration, and contents. Store in the refrigerator at 36-46°F to maintain stability Reconstituted IGF-1 LR3 typically lasts 4-5 weeks under these conditions, though some sources suggest up to potentially longer. Discard if any discoloration, precipitation, or odor develops.
It’s best practices for safety and efficacy to use fresh syringes to avoid cross-contamination, and understanding the peptide’s sensitivity to temperature, and aggressive handling.
This demonstration is purely for educational and research purposes—I’m not a medical professional, and this is not advice for personal use. Peptides can have powerful biological effects; always consult with a qualified healthcare provider or researcher before handling or experimenting.
Potential risks include improper dosing leading to hypoglycemia, injection site reactions, or unintended growth promotion, so proceed with caution and adhere to all local laws and ethical guidelines.
If you’re passionate about peptide science, biohacking, muscle physiology, or optimizing fitness through research-grade compounds, give this video a thumbs up, subscribe for more tutorials on peptides like BPC-157, TB-500, or GHK-Cu, and leave a comment below sharing your experiences or questions (research-related only, of course)!
#IGF1LR3 #PeptideReconstitution #BodybuildingScience #ResearchPeptides #BiohackingTips #MuscleGrowth #FitnessOptimization #PeptideTutorial #SterileTechniques #LabResearch
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🧬💉🧪Reconstituting IGF-1 LR3💪💉🧬
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