@Drew Griffin Important safety first — read this before you do anything: DMSO is a powerful solvent and a skin/chemical penetrant. It can carry whatever is dissolved through skin and into the bloodstream, can cause irritation, and must be sterile, pharmaceutical grade if you plan to use it in preparations that will be injected or come into contact with skin. Whenever possible, use bacteriostatic water or sterile saline per the manufacturer’s instructions. If you do not have explicit vendor guidance saying to use DMSO, strongly consider using bacteriostatic water instead and check with a qualified medical professional. Below is a careful, conservative method for dissolving SLU-PP-332 (5 mg) using sterile DMSO as an initial solvent, then diluting into bacteriostatic water to a safe final DMSO concentration. This method assumes aseptic technique and sterile, pharmaceutical-grade DMSO. Goal Make the peptide fully dissolved using a small amount of DMSO, then dilute to a final total volume of 2.0 mL so final concentration = 2.5 mg/mL, while keeping final DMSO ≤ 5% (safer for parenteral use). Supplies (must be sterile) - SLU-PP-332 vial (5 mg, lyophilized) - Sterile, anhydrous pharmaceutical-grade DMSO (single-use vial or ampoule) - Bacteriostatic water (sterile) - Sterile syringes (calibrated, e.g., 1 mL and 3 mL) and sterile needles - Alcohol swabs, sterile gloves, clean working surface (preferably laminar flow/aseptic area) - Labels and disposal container Conservative step-by-step (example that keeps final DMSO ≈ 5%) 1. Work aseptically. Wash hands, wear gloves, clean vial tops with alcohol swab. 2. Decide how much DMSO to use. Use a minimal volume that will dissolve the peptide — for 5 mg, try 0.10 mL (100 µL) DMSO as a starting point (this gives high solvent strength and keeps final DMSO low after dilution). 0.10 mL DMSO + 1.90 mL bacteriostatic water → total 2.0 mL; final DMSO fraction = 0.10 / 2.0 = 5%. 3. 4. Draw 0.10 mL sterile DMSO with a sterile 1 mL syringe. 5. Inject the 0.10 mL DMSO into the peptide vial slowly, aiming down the vial wall. Gently swirl or roll the vial to help dissolve. Do not shake vigorously. If the powder does not fully dissolve in 0.10 mL after gentle swirling (give it a minute), you may increase DMSO in small increments — but remember this raises the final % DMSO. 6. Once fully dissolved in DMSO, draw 1.90 mL bacteriostatic water with a fresh sterile syringe and slowly add it to the vial to reach 2.0 mL total volume. Mix gently by rolling. 7. Label the vial with final volume, concentration, DMSO % (e.g., “5 mg in 2.0 mL — 2.5 mg/mL — DMSO 5%”), date/time, and storage instructions. 8. Storage: keep refrigerated (per manufacturer guidance). If vendor instructions aren’t available, treat reconstituted peptide conservatively (short shelf life) and avoid prolonged storage; when in doubt, prepare fresh or consult the vendor/medical professional.