Here’s a video that was requested by one of our community members, @Rajesh N! 🙌🏽 We use the Gram stain all the time in microbiology, but do you understand WHY Gram-positive bacteria stay purple while Gram-negative bacteria turn pink? 👀 In this quick review, we’re breaking down the principle behind the Gram stain and what’s happening at each step: 🟣 Crystal Violet — Primary stain 🧪 Iodine — Mordant ⚪ Decolorizer — The critical step! 🩷 Safranin — Counterstain We’ll also look at: 🔬 Gram-positive vs. Gram-negative cell walls 🧠 Why the decolorization step matters ⚠️ What happens with over- and under-decolorization ❓ A quick knowledge check at the end! 🎥 Watch the video below and test yourself as we go! And remember if there’s a microbiology topic you’d like me to cover in a future video, let me know! You can always drop your questions or requests in the Specific Topics Help tab. 💬 Hope this helps make the Gram stain easier to understand instead of just memorize! 🧫💙