H²O² Hydrogen Peroxide use for Substrate
Hydrogen peroxide (HâOâ) can be useful in mushroom cultivation â but only in very specific, controlled applications. It is not a general-purpose sterilizer for colonized substrate. Used correctly, it can reduce contamination pressure and improve workflow hygiene. Below is a structured breakdown from a substrate and cultivation systems perspective. 1ď¸âŁ How HâOâ Works in Mycology Hydrogen peroxide breaks down into: HâOâ â HâO + Oâ (water + oxygen) It kills or damages: - Bacteria - Many mold spores - Yeasts However: - Actively growing mushroom mycelium produces catalase, which breaks down peroxide. - Spores do NOT have strong catalase protection â vulnerable. - Dormant contaminants are more sensitive than established mycelium. This is why timing matters. 2ď¸âŁ Practical Uses in Cultivation A) Surface & Equipment Sanitation Best use case. 3% HâOâ (standard pharmacy solution) can be used to: - Wipe tools - Clean work surfaces - Treat gloves during sterile work - Mist inside still air boxes â Leaves no residueâ Breaks down naturallyâ Mild compared to bleach B) Spore Germination Work (Agar Use) Low concentrations (0.1â0.3%) can be added to agar media to: - Suppress bacterial contamination - Allow mushroom spores to germinate - Improve success rate when working non-sterile â ď¸ Only useful at inoculation stage â once contaminants establish, peroxide wonât fix it. C) Soaking Fresh Substrates (Limited Use) Sometimes used in: - Straw pasteurization assistance - Short soak treatments to reduce surface mold spores Typical approach: - 0.1â0.3% final concentration - 30â60 minute exposure However: đŤ It does NOT replace proper pasteurizationđŤ It does NOT penetrate dense substratesđŤ It breaks down quickly in organic matter D) Treating Casing Layers Very dilute solution (0.1â0.2%) may: - Reduce surface mold on casing - Lower bacterial blotch risk But: - Overuse damages hyphae tips - Repeated application reduces pinning potential