Question:
The ZiehlāNeelsen stain is primarily used to detect:
ā
Correct Answer:
B) Acid-fast organisms
š§ Explanation:
The ZiehlāNeelsen stain is used to identify acid-fast organisms, especially:
š Mycobacterium species (like M. tuberculosis)
These organisms have a waxy cell wall rich in mycolic acids, which allows them to:
⢠Retain carbol fuchsin dye
⢠Resist decolorization with acid-alcohol
⢠Appear bright red/pink rods š“ under the microscope
š¬ What about the other options?
A) Fungal spores ā
⢠Typically seen with special fungal stains (e.g., lactophenol cotton blue)
C) Bacterial capsules ā
⢠Detected using India ink or capsule stains
D) Spirochetes ā
⢠Too thin for Gram stain ā seen with dark-field microscopy or special stains
š” Quick Recall Tip:
š„ Ziehl-Neelsen = Acid-Fast = Mycobacterium
š„ Think: Red rods = Acid-fast
š Did you get it right?
Drop a š„ or š§« below!